The Effect of HPSE on
Trophoblast Cell Proliferation and Apoptosis.
In early pregnancy, APL combines with autoantigens on the
trophoblast cells of the maternal-fetal interface.
And PAI-1 is regulated by TGF-[beta] in different kinds of cells, such as macrophages [22], renal tubular cells [23],
trophoblast cells [24], and hepatocytes [25].
Trophoblastic mitochondria are an important place for long-chain fatty acid [sz] oxidation during pregnancy, and abnormal long-chain fatty acid [sz] oxidation may induce mitochondrial damage in
trophoblast cells. In addition, a previous study reported that LPS can inhibit Bcl-2 expression and promote Bax and caspase-8 expression to induce apoptosis in
trophoblast cells via the mitochondrial pathway.
Thus, RANTES might cooperate in the maternal tolerogenic immune response to allow
trophoblast cell survival and migration [18].
Our results also demonstrated that miR-411, like miR-376c (shown before (22) and here), can promote
trophoblast cell proliferation, migration, and invasion.
Interplay of cAMP and MAPK pathways in hCG secretion and fusogenic gene expression in a
trophoblast cell line.
HTR-8/SVneo cells, which was derived from human invasive extravillous
trophoblast cells. The cells were cultured in Roswell Park Memorial Institute (RPMI)-1640 (Gibco, California, USA), containing 10% fetal bovine serum (FBS, Gibco); primary trophoblasts were isolated by trypsin-DNase I digestion.
For the RAS siRNA studies, transfection of
trophoblast cells was performed as previously described [35] using K-Ras siRNA (siKRAS), H-Ras siRNA (siHRAS), and negative control siRNA (siCONT) obtained from Ambion (Thermo Fisher Scientific; Scoresby, Vic, Australia).
Trophoblasts were assessed by immunocytochemistry for CK7 (
trophoblast cell marker) positivity.
Interferon-y inhibits extravillous
trophoblast cell invasion by a mechanism that involves both changes in apoptosis and protease levels.
Stepanova et al., "Changes in functional activity of JEG-3
trophoblast cell line in the presence of factors secreted by placenta," Archives of Medical Research, vol.