The Effect of HPSE on Trophoblast Cell
Proliferation and Apoptosis.
In early pregnancy, APL combines with autoantigens on the trophoblast cells
of the maternal-fetal interface.
And PAI-1 is regulated by TGF-[beta] in different kinds of cells, such as macrophages , renal tubular cells , trophoblast cells
, and hepatocytes .
Trophoblastic mitochondria are an important place for long-chain fatty acid [sz] oxidation during pregnancy, and abnormal long-chain fatty acid [sz] oxidation may induce mitochondrial damage in trophoblast cells
. In addition, a previous study reported that LPS can inhibit Bcl-2 expression and promote Bax and caspase-8 expression to induce apoptosis in trophoblast cells
via the mitochondrial pathway.
Thus, RANTES might cooperate in the maternal tolerogenic immune response to allow trophoblast cell
survival and migration .
Our results also demonstrated that miR-411, like miR-376c (shown before (22) and here), can promote trophoblast cell
proliferation, migration, and invasion.
Interplay of cAMP and MAPK pathways in hCG secretion and fusogenic gene expression in a trophoblast cell
HTR-8/SVneo cells, which was derived from human invasive extravillous trophoblast cells
. The cells were cultured in Roswell Park Memorial Institute (RPMI)-1640 (Gibco, California, USA), containing 10% fetal bovine serum (FBS, Gibco); primary trophoblasts were isolated by trypsin-DNase I digestion.
For the RAS siRNA studies, transfection of trophoblast cells
was performed as previously described  using K-Ras siRNA (siKRAS), H-Ras siRNA (siHRAS), and negative control siRNA (siCONT) obtained from Ambion (Thermo Fisher Scientific; Scoresby, Vic, Australia).
Trophoblasts were assessed by immunocytochemistry for CK7 (trophoblast cell
Interferon-y inhibits extravillous trophoblast cell
invasion by a mechanism that involves both changes in apoptosis and protease levels.
Stepanova et al., "Changes in functional activity of JEG-3 trophoblast cell
line in the presence of factors secreted by placenta," Archives of Medical Research, vol.