Oxidized TPO and Tg elicit an autoimmune reaction with production of antibodies against these altered proteins with subsequent damage to the apical membrane of the thyroid cells
, resulting in the lymphocytic infiltration and in the clinical manifestations of Hashimoto's thyroiditis.
Because perchlorate inhibits iodide uptake, we focused on the genetic defects causing CH involving the iodination process of the THs, particularly a) defects in iodide transport from circulation into the thyroid cell
; b) defects in iodide transport from the thyroid cell
to the follicular lumen, often combined with inner ear deafness [Pendred syndrome (PDS)]; and c) defects of iodide organification.
(11) TSAb can mimic thyrotropin action and stimulate thyroid cells
. On the contrary, TSH-blocking antibodies (TSHBAb) can bind to the TSH-R and induce a block of the TSH-mediated activation of thyroid cells
Purified hCG, like TSH, has been shown to (a) increase iodide uptake and cAMP production in FRTL-5 rat thyroid cells
; (b) increase cAMP production dose-dependently and displace binding of [sup.125]I-labeled TSH in Chinese hamster ovary cells stably transfected with human TSH receptor; and (c) stimulate iodide uptake, or ganification, and [T.sub.3] secretion in cultured human thyroid follicles (17, 18).
Zannini, "Identification of novel Pax8 targets in FRTL-5 thyroid cells
by gene silencing and expression microarray analysis," PLoS One, vol.
Thyroid-stimulating hormone stimulates increases in inositol phosphates as well as cyclic AMP in the FRTL-5 rat thyroid cell
Improvement from immune collapse in patients with HIV may induce the appearance of thyroid autoantibodies and AITD and treatment of hepatitis c with interferon alpha has had similar effects likely secondary to a direct influence on the thyroid cell
Excess iodide alone can be toxic to thyroid cells
in culture and cause thyroid hypertrophy and changes in colloid in vivo in the rat model (Capen 1997; Vitale et al.
More recently, we have reported the detection of circulating thyroid cells
in whole blood samples of individuals with no known thyroid disease, using a qualitative RT-PCR assay (3).
The rat NIS (rNIS) was the first member to be cloned by functional screening of a human cDNA library from FRTL-5, a rat thyroid cell
line, in Xenopus laevis oocytes (7).
TTF1 and PAX8 are implicated in proliferation and survival of thyroid cell
precursors, and TTF2 plays a role in migration and proliferation of thyroid precursors.
If high iodide status is continuous, iodine transportation into the thyroid cell
decreases because of the decreases in sodium iodide symporter (NIS) mRNA, NIS protein , and NIS uptake.