A
radiolabeled primer (much like a PCR primer) is annealed down at one end of the sequence, and a DNA polymerase is then used to synthesize a new complementary strand to the target material.
We stopped the PCR reaction at various cycle numbers to establish the linearity of amplification, resolved the
radiolabeled PCR product on an agarose gel, excised the band from the gel, and measured the radioactivity.
(1) Once the
radiolabeled isotopes are produced its replacement can be carried out via isotopic substitution or non isotopic substitution.
In general, temperate forest ant species do not have nests or middens deeper in the soil than would be detectable by our
radiolabel and probe (J.
For quantitation of
radiolabel, aliquots of at least 0.1 mL were obtained from solutions collected at particular steps in the separation procedure.
The number of DPM incorporated by females was expected to depend, in part, upon the extent of
radiolabel incorporation by their mates.
The study demonstrates that
radiolabeled dextran cysteamine mannose might prove a successful radiopharmaceutical for sentinel lymph node detection in future clinical applications.
However, modern APIs and their delivery methods are becoming increasingly more complex resulting in synthetic challenges for making these
radiolabeled products.
In this proof of concept study, we synthesized and evaluated methods to
radiolabel the depsidomycin derivative DOTA-TBIA101 with [sup.68]Ga.
1.) Children presenting with low-grade fever and back pain who appear clinically to have vertebral osteomyelitis and whose planar bone scans reveal focal
radiolabel uptake may actually suffer from acute, noninfectious discitis.
Positron emission tomography (PET) imaging agent MVT-2163 combines a well-established PET imaging
radiolabel, Zirconium, with the targeting specificity of the company's HuMab-5B1 antibody.