Estrogen and phenol red
free medium for osteoblast culture: Study of the mineralization ability.
Katzenellenbogen, "Phenol red
in tissue culture media is a weak estrogen: implications concerning the study of estrogen-responsive cells in culture," Proceedings of the National Academy of Sciences of the United States of America, vol.
Two additional metal indicators (phenol red
and GelRed[TM]) were added before LAMP reaction but UV transilluminator was required.
After 24 h, the medium was replaced for phenol red
and hormone-free DMEM medium.
These treatments produce lowering of TEER values (Figures 3(a) and 3(b)), highlighted by increased permeability of TJs (phenol red
) and by alteration of epithelium.
Expectorant activities were studied by measuring the amount of phenol red
secretion in mice tracheas, and the results are given in Fig.
The samples were rinsed in cold serum-free DMEM-F12 without phenol red
(Lonza BioWhittaker) and supplemented with 1% of penicillin/streptomycin (Sigma-Aldrich) and with 1% of L-glutamine (Sigma-Aldrich), to remove excessive blood.
The carboxyl groups content (CC) was determined according to Pohl's method  by titrating the solution of PET in benzyl alcohol/chloroform with standard NaOH in benzyl alcohol in the presence of phenol red
as an indicator.
They were used in experiments where cells were grown in the lab for five to six days, with or without exposure to a growth-stimulating oestrogen medium called phenol red
. Cells cultured without the growth medium differentiated into immature small cells of various different types
The addition of phenol red
to the agar medium indicates an alkaline shift in the presence of growth, turning the medium from yellow to pink.
New techniques were developed including a phenol red
dextrose broth-metabolic inhibition test (PRDB/ MIT) that enable large-scale and rapid screening for transconjugants.
Phenol red-free media were used, given findings that phenol red
has weak estrogenic activity X15]; charcoal-stripped fetal bovine serum is devoid of any steroid hormones.