Expectorant activities were studied by measuring the amount of phenol red
secretion in mice tracheas, and the results are given in Fig.
dextrose broth (PRDB) was tested as a potential metabolic inhibition test (MIT) and selective medium, when supplemented with antibiotics to differentiate bacterial phenotypes.
Measurement of secretolytic activity was evaluated by measuring the excretion of phenol red
into respiratory tract (Coppi and Gatti 1989).
A complete ocular examination including modified Schirmer tear test I (mSTTI) (Schirmer tear test, Intervet, Summit, NJ, USA) (n = 9 birds; 18 eyes) or phenol red
thread test (PRTT) (Zone-Quick, Showa Yakuhin Kako Co, Tokyo, Japan) (n = 8 birds; 16 eyes), fluorescein staining, and intraocular pressure (IOP) evaluation by applanation (Tonopen XL [TP] Mentor Corp, Santa Barbara, CA, USA) (n = 9 birds; 18 eyes) or rebound (TonoVet [TV] Tiolat Oy Lumic International, Baltimore, MD, USA) (n = 8 birds; 16 eyes) tonometry followed by slit-lamp biomicroscopy, and direct ophthalmoscopy was performed.
Key words: eye, tear production, PRTT, phenol red
thread test, avian, common mynah, A cridotheres tristis
Each well of a sterile 96-well flat-bottomed, microtitre plate was inoculated with 100 [micro]l of media, one plate with enriched PPLO medium with glucose and phenol red
and the other without.
For strain cultures, cells were grown in phenol red
containing Dulbecco's modified Eagle medium, 1 g/L glucose, supplemented with 5% fetal calf serum (FCS), and 1% antibiotic (penicillin/streptomycin) in a 5% C[O.
Key words: phenol red
thread test, tear, ophthalmology, Falconiformes, avian, Falco
thread tear test (PRT) was performed to evaluate tear production as described in a previous study.
Cells were then washed with medium three times and resuspended in RPMI-1640 without phenol red
for the N-acetyl-[beta]-D-hexosaminidase activity release assay or in complete DMEM for the cytokine secretion assay.
Although a Schirmer tear test or a phenol red
thread test was not performed on the left eye, adequate tear production was estimated because of the healthy glistening appearance of the left cornea.
Blood agar base,urea broth agar,simmons citrate agar,agar lia,common mannitol salt agar phenol red