Subsequently, cells were fixed in 4% paraformaldehyde
for 30 min at 4[degrees]C.
At the end of the experiment, rats were sacrificed, and the right eyeballs were removed and then fixed with 4% paraformaldehyde
in phosphate-buffered saline.
To observe the shape of nucleus, cells were fixed using 4% paraformaldehyde
followed by washes with PBS.
For fluorescence observation, cells were fixed with 4% paraformaldehyde
in HBSS for 15 min at RT and afterwards rinsed with PBS (3x5 min, RT).
Efficacy was measured on a log scale and 6-log spore reduction in spores was observed after one hour at 55[degrees]C and after 12 hours at room temperature, making MeI a viable alternative to current fumigation techniques such as chlorine dioxide, vaporous hydrogen peroxide, paraformaldehyde
, and MeBr.
Gill fragments were dissected, washed in saline buffer and fixed immediately in Bouin, 4% paraformaldehyde
and Karnovsky solution (paraformaldehyde
75% and glutaraldehyde 25%) for 24h.
Briefly, the embryos and pups were incubated in the X-gal reaction mixture for 24 hr at room temperature, then fixed in 4% paraformaldehyde
at 4[degrees]C for 24 hr, and subsequently transferred to 70% alcohol.
Recently, we  were able to synthesized a new type of benzoxazine aminepropyltrihydroxylsilane (Bz[(OH).sub.3]) monomer using bisphenol A, paraformaldehyde
4-t-butyl cyclohexanone, piperidine, paraformaldehyde
, acetic acid, hydroxyl amine hydrochloride, sodium acetate, sodium bisulfite, potassium cyanide, diethyl ether and other chemicals were purchased from Merck chemical Co.
Monkeys feel pain and fear just as we do and their instincts - like ours - are to be free and protect their own lives, not to be locked in a cage inside a lab, have brains removed or sliced open, endure tremors, loss of voluntary movement or incapacity or be pickled in paraformaldehyde
while their hearts are beating.
P falciparum infected RBCs were mixed with 1% paraformaldehyde
solution in PBS buffer at various concentrations and stored at 4[degrees]C for 30 min.
A rectangular piece of skin situated on the dorsum, 2 cm from the base of the tail, was fixed in paraformaldehyde
and processed for subsequent histological examination.