The
paraffin sections were then incubated with biotinylated secondary goat anti-rabbit IgG for 30 min followed by horse radish peroxidase-conjugated streptavidin (BD Pharmingen, USA) and diaminobenzidine staining; an irrelevant rabbit IgG was used for negative control.
An immunohistochemical evaluation of progesterone receptor in frozen sections,
paraffin sections, and cytologic imprints of breast carcinomas.
Nevertheless,
paraffin section IHC stains for [kappa], [lambda],and sometimes the immunoglobulin heavy chains are useful in evaluating potential lymphoid or plasmacytic neoplasms, especially if there is plasmacytoid differentiation or overt plasma cells.
Our study demonstrated relative specificity of c-Kit as a marker of early hematopoietic cells committed to the myeloid lineage in the
paraffin sections. It can be especially helpful to differentiate these cells from lymphoid cells in most cases.
Therefore, fluorescent markers of monocytes can be detected easily on bone marrow
paraffin sections of AML with reticulin myelofibrosis.
Approximately 5mm thick
paraffin sections were stained with hematoxylin and eosin (H&E) for morphological observation.
Immunohistochemistry (IHC) with an antihuman CD90 antibody was performed to track the MSCs in
paraffin sections.
Paraffin sections of the skin, oral mucosa, duodenum, pancreas and mesenteric lymph nodes were stained with Grocott's methenamine silver stain (GMS) for the identification of Pythium insidiosum.
Paraffin sections of the biopsy tissue consisted of fragments of trabecular bone.
Paraffin sections were used for histology and frozen sections for immunofluorescence and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) as described below.
We designed the present morphological study in rats by combining intravascular injections of multifunctional contrast materials, "real-time" digital radiography (DR) for microangiogram [14], histomorphology of cryotome and
paraffin sections with special and standard stains, and mathematical assumptions in an attempt to experimentally elucidate the pathways of LPD trafficking through the hepatic microcirculation.
Two weeks later, the mice were sacrificed; part of the aortic tissue was used to prepare
paraffin sections and the other part was analyzed as a homogenized supernatant.
