When interpreting WR samples, one should look at the whole profile of HBV tests, including the HBsAg neutralization test
and assays of other hepatitis serologic markers, to provide the most accurate status of HBV infection to clinicians.
In the neutralization test
, 16 of 19 paired serum samples showed seroconversion ([greater than or equal to] 4-fold increase) to the MI-110 strain of Getah virus (Table).
Focus also plans to start offering in its laboratory a confirmatory Flavivirus (WNV) plaque reduction neutralization test
(PRNT) by Summer 2003.
Virus Titration and Plaque Reduction Neutralization Test
The gold standard laboratory test, the Plaque Reduction Neutralization Test
(PRNT), has been used to determine the ability of the antibodies to neutralize the infectivity of the virus in an individual following vaccination.
The presence of ONNV-specific neutralizing antibodies in the second serum sample was confirmed by a virus neutralization test
To determine the presence of neutralizing antibodies in the serum samples, we used an orthopoxvirus 70% plaque-reduction neutralization test
as described (4).
000 RFU as saturation signal; VNT, virus neutralization test
(highest neutralizing serum dilution indicated); NT, not tested; --, negative.