These results are complemented by those of the study of the inhibitory effect and the neutralization test
of organic acids in the presence of organ extracts that show the capability of recuperating the strain at test doses of 0.625% and 1.25% for the three inoculums and in different media for lactic acid.
All viruses were used in the neutralization test
at a concentration of 100TCID50.
A study of 9 Thai patients with documented recent DENV infections found all sera were cross-reactive with ZIKV, including when tested using neutralization tests
, in the absence of current or prior ZIKV infection.
(1.) Oh J S, Song D S, Yang J S, et al.: Comparison of an enzyme-linked immunosorbent assay with serum neutralization test
for serodiagnosis of porcine epidemic diarrhea virus infection [J].
The RFFIT is one of the most widely used substitutes to the Mouse Neutralization Test
(MNT) and is a rapid test that requires only 20 hours for completion and is slightly more sensitive than the MNT for detecting virus neutralizing antibodies (VNAs) in postvaccinal sera [35, 36].
Serum neutralization test
(SNT) To determine the antibody status of herds against BVDV, serum samples were subjected to Serum neutralization test
as described by Deregt et al.
perfringens strains were then typed for their toxigenicity and typing by the mice neutralization test
and dermonecrotic test in guinea pigs (Murray et al., 2003).
Serum specimens were tested at the Centers for Disease Control and Prevention (Fort Collins, CO, USA) by IgM antibody capture ELISA (MAC-ELISA) for detection of Zika virus and dengue virus IgM and by plaque reduction neutralization test
(PRNT) to detect Zika virus and dengue virus neutralizing antibodies (5,6,22).
When positive or inconclusive results occur, additional testing (plaque reduction neutralization test
) to confirm the presence of antibodies to Zika virus will be performed by the CDC or a CDC-authorized laboratory.
Bovine Viral Diarrhea Virus (BVDV) antigens and antibodies in samples were investigated with Antigen Capture ELISA (ACE) and Serum Neutralization Test
In the case of tests for HBsAg, manufacturers provide a neutralization test
that can be used to confirm true positivity of the results.