2, to dilute both red cell hemolysates and folic acid calibrators before microbiological assay
, whereas we used 5 g/L sodium ascorbate (25 mmol/L).
The microbiological assay is more tolerant of the presence of polyglutamate RCF forms and will tend to underestimate total folate where hemolysis is complete but deconjugation is not.
Results with commercial radioassay kits compared with microbiological assay of folate in serum and whole-blood.
7) adjusted with 1 mol/L NaOH to a variety of pHs, either without (conventional assay) or with added saponin, were assessed after 2-h incubation at 37 [degrees]C (to allow for erythrocyte folate polyglutamate deconjugation) by microbiological assay with L.
The microbiological assay was carried out on microtiter plates with the chloramphenicol-resistant strain of Lactobacillus casei , and the radioassay was performed with the Dualcount Solid Phase Boil assay (Diagnostic Products Corp.
Table 1 shows that, overall, the microbiological assay gives higher values than the radioassay, as reported in the literature [2, 11].
It is also possible, but less likely, that the microbiological assay is underestimating the concentration of some active folate.
A variety of disposable vials can be used to perform several microbiological assays
, including total aerobic count (TAC), Yeast and Mould (YM), coliform (CM), E.
Currently, the major analytical approaches used for folate determination are microbiological assays
, radioassays, chemiluminescence assays, HPLC, and ion-capture assays.