Nonstandard abbreviations: tHcy, total homocysteine; holoTC, holotranscobalamin; TC, transcobalamin; TCN2, transcobalamin 2 gene; MBA, microbiological assay
; and MMA, methylmalonic acid.
Whole-blood folate values in subjects with different methylenetetrahydrofolate reductase genotypes: differences between the radioassay and microbiological assays
. Clin Chem 1998;44:186-8.
Folate analysis by microbiological assays
fulfills two important requirements for such screening by providing an acceptable detection limit and specificity at a low reagent cost.
We evaluated these findings using our "in house" microbiological assay
(2) and fresh clinical EDTA-WB samples.
The degree of hemolysis and/or the completeness of deconjugation will obviously influence the estimation of erythrocyte folate concentrations, but may affect the accuracy and stability of the classical biological method (microbiological assay
) and the now widely used competitive folate-binding protein assays (radioassays and immunoassays) differently.
The microbiological assay
was carried out on microtiter plates with the chloramphenicol-resistant strain of Lactobacillus casei , and the radioassay was performed with the Dualcount Solid Phase Boil assay (Diagnostic Products Corp.), which was that used by van der Put et al.
undertaken to check on bacterial populations--coliform, psychrotrophic, aerobic mesophilic and Salmonella populations--were not significantly different in the pea protein concentrate-formulated sausage and the control.
In addition to information obtained directly from the patient during consultation, the diagnosis of syphilis normally includes clinical examination and serological and microbiological assays
. As a rule, biopsy is only required when lesions do not subside completely.
This project will adopt a multidisciplinary approach where biotransformation assays, kinetic modelling and high-throughput microbiological assays
will be integrated to provide a thorough understanding of temperature dependence of micropollutant biotransformation kinetics in aerobic biological systems, taking into account the strong relationship between taxon traits and the environment.
To overcome these limitations, qPCR-based methods and classical microbiological assays
should be used simultaneously to obtain more data from the factory.