(1) The underlying cytogenetics is due to translocation t(11;14)(q13;q21), which eventually results in overexpression of cyclin D1, a cell cycle protein not usually expressed in
lymphoid cells; therefore, disrupting the central cell cycle pathway balance resulting in MCL.
B, Biopsy shows sheets of
lymphoid cells that replaced much of the dermis and are separated from the epidermis by a grenz zone.
The aspirate sample was stained with Papanicolaou stain, and results showed groups of small to medium
lymphoid cells with a background of erythrocytes.
The aspiration revealed hypocellular marrow with 50% of atypical small to medium sized
lymphoid cells. Because of the lack of specific antibodies, bone marrow biopsy was performed and based on immunohistochemical profiles (T lymphocytic cells positive for CD2, CD3, CD5, CD8, granzyme and TIA and negative for hairy cell markers, CD10, MUM 1, bcl 1, CD4 and CD56), the diagnosis of T-LGLL was established.
Immunohistochemistry revealed CD3: positive; CD4: small number of
lymphoid cells positive; CD8: majority of the
lymphoid cells positive; CD20: positive; CD30: negative, this report confirmed the diagnosis of LyP.
When the smears showed a monotonous population of
lymphoid cells with mainly rounded nuclei slightly larger than those of normal small lymphocytes and many mitotic figures were taken as suggestive of non-Hodgkin's lymphoma (NHL).
(d) The neoplastic
lymphoid cells in the coronary artery are positive for CD20 (H&E, x200).
Caption: Figure 4: (a)
Lymphoid cells invading sinusoidal spaces (HE 200x); (b) diffuse lymphoid infiltration (HE 200x); (c) nodular lymphoid aggregate (HE200x); (d) area of steatosis (HE100x).
Caption: Figure 3: Hematoxylin-eosin stain of a section through the center of the nodule (a) shows numerous confluent lymphoid follicles composed of large, reactive lymphocytes with intervening smaller
lymphoid cells. An immunostain for the B cell marker CD20 (b) shows that the follicles are composed of B cells, while the stain for the T cell marker CD3 (c) shows that the mantle zone and interfollicular areas have predominantly T cells, typical of benign lymphoid tissue.
In addition, RA induces the homing of innate immune cells, such as innate
lymphoid cells (ILCs) [8] besides regulatory and effector T and B cells, to the gut [9-11].
van Ree, "Type 2 innate
lymphoid cells: at the cross-roads in allergic asthma," Seminars in Immunopathology, vol.
BCR-ABL1 positivity in both myeloid and
lymphoid cells, rather than just in the lymphoid component, as it was detected in our case, would support the diagnosis of CML in T-cell blast crisis [9].