This agent is reported to cause specific damage, that is, liver damage, with no effects on the other tissue or organs, which could be accounted for by the fact that the liver cells
contain high levels of galactokinase and galactose-1-p uridiltransferase (Keppler and Decker, 1969).
Ultrastructural examination demonstrated increase in the number of mitochondria, and the liver cell
cytoplasm was crowded with mitochondria (Fig.1).
Cytonet says its liver cell
therapy has the potential to stabilize ammonia levels through infusion of healthy liver cells
, potentially increasing the length of time children can safely wait until they can undergo a liver transplant.
And liver cells
don't survive in typical cell cultures, confounding scientists who want to grow them in the lab.
The ground-breaking research on the liver cell
therapy is currently being developed by the Cytonet in partnership with the University Hospital of Heidelberg.
Clinical trials of combination antioxidants, including one with silybin (milk thistle) and phospholipids showed good results at improving insulin resistance and reducing markers of liver cell
fibrosis (a finding in advanced liver disease).
The Fa2N-4 and Ea1C-35 immortalized human hepatocyte cell lines were derived from normal human liver cells
, and are nontumorigenic, stable in culture, and produce therapeutic plasma proteins in cell culture.
The protection of liver cells
against toxic materials including drugs, lipid peroxidation and free radical injury may decrease inflammation, improve liver blood flow and ultimately help in reduction of further liver damage (Jiang 1997).
The cytotoxic effects observed by exposing rat liver cell
to arsenic trioxide depicted different observations; PFT-[alpha] did provide protection to rat liver cells
upon exposure to arsenic trioxide: L[C.sub.50s] were 670 [+ or -] 8.15 [micro]g/ml and 573 [+ or -] 26.2 [micro]g/ml with and without PFT-[alpha] respectively (Fig 2).
"Equally important, I consider HepaLife's PICM-19H cell research of particular significance since it directly addresses the widely-held scientific opinion that the greatest hindrance in development of a therapeutically effective artificial liver device has been the lack of an appropriately defined liver cell
line, able to provide the functions of an intact liver," Soheili concludes.
The technique, developed by a team at King's College Hospital, involves injecting healthy donor liver cells
into the unhealthy livers of patients, inducing the patient's liver cells
to regenerate, replacing dysfunctional cells with new healthy ones.
According to "The Complete German Commission E Monographs: Therapeutic Guide to Herbal Medicines," the active ingredients in milk thistle prevent toxins from penetrating liver cell
membranes and stimulate the formation of new liver cells