Prediction of isoelectric points
of legume oilseed proteins from their amino acid composition.
CALIBRATED IMA QUANTIFIES PROTEINS WITH DIFFERENT MOLECULAR WEIGHTS AND ISOELECTRIC POINTS
The isoelectric point
(pI) value was estimated for the purified camel liver G6PD at 6.6-6.8 (Figure 4(d)).
Properties such as isoelectric point
, log P (octanol-water partition coefficient), log S (pH dependent aqueous solubility), and water solubility of oligopeptides were calculated, which shows that PGKYKLAVSEK (derived from motavizumab and glycoprotein A interaction), SGVQPDAPSNDSKDT (derived from motavizumab and fusion protein interaction), PSTLSVSSGTVYEKHEGLS (derived from motavizumab and matrix protein interaction), and KREAKQEE (derived from motavizumab and small hydrophobic protein interaction) have good water solubility while all others show poor water solubility (Table 2).
The isoelectric point
of bare Fe NPs was about 8~8.5 [31, 32].
The deduced primary structure of the Cashmere goat ribosomal protein S6 consists of 249 amino acids, and the predicted molecular weight is Mr 28,785 Da for the unmodified protein, the estimated isoelectric point
(pI) is 11.13.
In practice, it is more common to use the pH of the isoelectric point
(IEP), which is the pH at which the zeta potential is zero [21-24].
Lamas MC, Torres Sanchez RM (1998) Isoelectric point
of soils determined by the diffusion potential method.
This observation is consistent with the previously reported results for the thermal aggregation of proteins close to their isoelectric points
, which showed the formation of monodisperse particulates .
ACO40483) is composed of 151 aa with a molecular weight of 17.43 KDa and an isoelectric point
# E.V E.N.V (Spot) 23-25 [marca] - 36-38 [marca] - 75 [marca] - 76 [marca] - 96 [marca] - 97 [marca] - 132-144 [marca] [marca] 148-149.1 [marca] - 150 [marca] - 151-160 [marca] [marca] 184-190 [marca] - 203 - - 205 - - 208 [marca] [marca] 215 [marca] - 219 [marca] - 223 - - MW = Molecular Weigth, Ip = Isoelectric point
, EV = Viable embryo, NV = Non viable embryo, [marca]: Present; -: absent.
Numerous factors affect protein binding, including pH and protein isoelectric point
; temperature; salts; surfactants; and other proteins, and hydrophobicity/hydrophilicity of those proteins.