Elongation of the linker region with parts of the mucin-like stalk of C[X.sub.3]CL1 and also deletion of domain II of PE reduced the efficacy of the FTPs in killing US28-expressing cells compared to F49A-FTP This is consistent with previous studies where eliminating the furin cleavage site by deletion or preventing cleavage with a point mutation in the sites reduced the cytotoxicity of a series of immunotoxins
Xiang et al., "A flow cytometry method to quantitate internalized immunotoxins
shows that taxol synergistically increases cellular immunotoxins
uptake," Cancer Research, vol.
Finally, we purified the immunotoxin
by nickel affinity chromatography and then verified the protein through SDS-PAGE (Figure 1(a)).
In addition, studies using immunosuppressant regimens along with the immunotoxins
also showed some benefit.[sup],, Other regimens, including a lymphocyte-depleting regimen, which consists of pentostatin and cyclophosphamide, were also found promising in delaying the stimulation of neutralizing anti-immunotoxin antibodies, thus allowing repetitive immunotoxin
treatments for patients with solid tumors.[sup] Pentostatin and cyclophosphamide selectively suppress the effect of T- and B-cells while largely sparing myeloid cells.[sup]
Survival Rate of ipRGCs after Immunotoxin
Wang, "Recombinant immunotoxin
therapy of solid tumors: challenges and strategies," Journal of Basic and Clinical Medicine, vol.
According to the researchers, these findings, coupled with results from previous studies, suggest that treating certain HIV-infected people with a combination of antiretrovirals and an immunotoxin
might help achieve sustained disease remission, in which HIV can be controlled or eliminated without a lifetime of antiretroviral therapy.
Talpaz et al., "Phase 1 study of an anti-CD33 immunotoxin
, humanized monoclonal antibody M195 conjugated to recombinant gelonin (HUM195/rGEL), in patients with advanced myeloid malignancies," Haematologica, vol.
Chapter 9 deals with the use of CD30 receptor as a target for immunotoxin
Inhibition of microglial activation after spinal injury reduces pain-related behaviors [44,91], and treatment with minocycline or the Mac-1-SAP immunotoxin
reverses morphological changes in microglia and attenuates functional and behavioral consequences of SCI.
Improvement of a recombinant anti-monkey anti-CD3 diphtheria toxin based immunotoxin
by yeast display affinity maturation of the scFv.
United States Department of Health and Human Services (Washington, DC) has patented recombinant immunotoxin
fusion proteins composed of the Fv domains of antibodies fused to bacterial or plant toxins.