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Determination of protein content: The erythrocyte membranes were assayed for protein content by the method of Lowry et al.19
A reduction in the SPM maintenance in erythrocyte membranes leads to a decrease in protective properties of erythrocytes, the acidotic shifts in blood plasma, a change to electrostatic properties in red blood cells and an increase in permeability of a membrane.
The total glutathione (GSSG + GSH) amounts inside the erythrocyte have been analyzed by a commercial kit (Glutathione Assay Kit, Sigma-Aldrich) following the instruction supplied by the seller.
A study39 showed that an increased RDW is significantly and positively associated with decreased erythrocyte deformability (p<0.003).
21 Erythrocyte lysate and [H.sub.2][O.sub.2] were added to phosphate buffer.
There are studies conducted on hormones (Cinar and Selcuk, 2005; Comba and Cinar, 2016), electrocardiogram (Kilicalp at al; 2004) under exposure to NaF, however no study was performed on erythrocyte osmotic fragility and parameters under concomitant exposure to fluoride and DMBA in the long term.
The rate of red blood sedimentation (ESR) was measured by the distance erythrocyte had sedimented in scale from top of the hematocrit tube and were enumerated as mm/h.
Basigin is a receptor essential for erythrocyte invasion by Plasmodium falciparum.
These parameters with their respective reference values (third trimester of gestation) included erythrocyte count (RBC), 2.71-4.43 x [10.sup.6]/[mm.sup.3]; hematocrit (Ht), 28.0-40.0%; hemoglobin (Hb), 9.5-15.0 g/dL; mean corpuscular volume (MCV), 81-99 fL; mean corpuscular hemoglobin (MCH), 29-32 pg/cell; mean corpuscular hemoglobin concentration (MCHC), 31-36 g%; red-cell distribution width (RDW), 12.7-15.3%; platelet count (Plt), 146-429 x 10.sup.3]/[mm.sup.3]; and mean platelet volume (MPV), 8.2-10.4 fL [37, 38].
On the smears made from day-2 EDTA sample, alterations in leukocyte and erythrocyte morphology were already evident; they were more pronounced on smears made on subsequent days.