The liver microtissues and embryoid bodies
have a diameter of about half a millimeter and are placed in different compartments connected through microchannels to guarantee a constant liquid exchange between the different groups of cells.
After hanging drop, the cells were aggregated and composed embryoid bodies
Zhang, "Engineering strategies for the formation of embryoid bodies
from human pluripotent stem cells," Stem Cells and Development, vol.
Temporal changes in the carbohydrates expressed on BG01 human embryonic stem cells during differentiation as embryoid bodies
. Glycoconj J 2008; 25(2): 121-36.
However, cardiac embryoid bodies
seemed more similar to their undifferentiated precursors than to the neural precursors, but RAGE level in their differentiated progenies, as in fibroblast and endothelial cells decreased similarly to the CML.
Movahedin, "Formation of embryoid bodies
from mouse embryonic stem cells cultured on silicon-coated surfaces," Cytotechnology, vol.
To encourage differentiation into neural cells, the researchers tried converting the iPSCs directly into neural stem cells as neurospheres--culture systems of free-floating neural cells--instead of embryoid bodies
that contain cells of other germ layers.
ES-like cells forming typical embryoid bodies
(EBs) after 5 days were transferred to confocal dishes coated with 0.1% gelatin and allowed to further differentiate for 2 to 3 weeks.
Because the new growth surface has low adherence, the pluripotent stem cells self-aggregate to each other rather than the plate surface, forming spheres termed embryoid bodies
. The size of the embryoid body influences the differentiation process, so it is important to control this variable when optimizing differentiation conditions.
Efficacy of solvent extraction methods for acellularisation of embryoid bodies
. Journal of Biomaterials Science, Polymer Edition.
Retinoic acid (vitamin A) is known to induce the differentiation of pluoripotent cells that result onto the formation of embryoid bodies
. To identify the differentiation potential of HFB-ES, cells were treated with trans-retinoic acid and examined under microscope for the embryoid body formation after 3 weeks of incubation.
(EBs) were formed by growing hES cells in suspension in a low attachment culture plate with Knockout DMEM/F12 media (Gibco, Life Technologies) supplemented by 20% knockout serum replacement, nonessential amino acids (0.1 mM), GlutaMAX (2mM), and beta-mercaptoethanol (0.1 mM).