Embryonic stem cells can form three-dimensional structures called embryoid bodies
and can differentiate spontaneously into a variety of cell types [5-7].
Salk researchers will initially utilize LEAP for non-destructive counting and characterization of embryoid bodies
, passaging of stem cell colonies and removal of unwanted spontaneously differentiating areas, and derivation of new induced pluripotent stem cell lines.
Embryoid body formation and in vitro differentiation Evaluation of the differentiation of porcine ES-like cells was conducted by spontaneously prolonged culture and in hanging drops as embryoid bodies
Clusters of mouse embryonic stem cells called embryoid bodies
more closely approximate true embryos in organization and structure than previously thought, according to researchers at the Stanford University School of Medicine.
These include mTeSR[R]1, the most widely used, feeder-independent maintenance medium for hESC and hiPSC, and AggreWell[TM], a unique tool to prepare size-controlled, uniform embryoid bodies
when differentiating these cells.