A, Sequencing results of the putative furin cleavage site
[sub.742][RNRR.sub.745] of the wild-type Sema3C and R745A mutant.
The CLV_PCSK_PC1ET2_1 motifs matched with amino acid residues KRF (175-177) residues with the functional NEC1/NEC2 cleavage site
(Lys-Arg-|-Xaa) present in the extracellular and Golgi membrane.
Peeters, "Effect of fusion protein cleavage site
mutations on virulence of Newcastle disease virus: Non-virulent cleavage site
mutants revert to virulence after one passage in chicken brain," Journal of General Virology, vol.
Alignments of potential cleavage sites
(a), two independent copper binding sites (b and c), and two C-terminal conserved motifs (d) are shown.
Vitellogenin proteins contain a lipid-binding domain near the N-terminus, a Domain of Unknown Function 1943 (DUF1943), a von Willebrand Factor Type D domain (VWD), and conserved RXXR furin/subtilisin-like protease cleavage sites
(Smolenaars et al., 2007; Prowse and Byrne, 2012).
Brennan, "Preparation of recombinant thioredoxin fused N-terminal proCNP: analysis of enterokinase cleavage products reveals new enterokinase cleavage sites
," Protein Expression and Purification, vol.
The enzyme was shown to hydrolyze oxidized insulin B chain most rapidly, followed by glucagons, substance P and Lys-Pro-Ile-Glu-Phe*Phe([NO.sub.2])Arg-Leu (*, cleavage site
) at pH 4.2 and 18[degrees]C.
An additional consideration is whether the cleavage site
has two or more potential cleavage sites
in a row, also known as "ragged ends" .
This stalk is open and accessible and contains the cleavage site
. This has led to the notion that sheddases function like lawnmowers that move over the surface of the cell and cut any protein stalks in their path.
The secreted protein of 227 amino acids has a proteolytic cleavage site
between [sup.179]Arg and [sup.180]Ser which when cleaved produces inactive fragments.
After size-fractioning of products in agarose gels, each product was purified (Wizard[R] SV gel and PCR clean-up system --Promega) and cloned into pGEM-T Easy Vector Kit (Promega), which has a plasmid of 3015 bp as cloning vector and a cleavage site
for each of the restriction enzymes used in the analysis (in the linker), including two EcoRI sites flanking the PCR product cloning site.