1B) may correspond to furin/subtilisin-like protease cleavage products
, which would be detected by this antibody (Appendix Fig.
The researchers took a closer look at a key protein in Alzheimer's pathogenisis, APP, and one of its cleavage products
One way of bypassing this lack of information has been introduced as a "processing-independent assay," which simply quantifies 1 in vitro cleavage product
that represents all secreted precursor molecules on a molar basis (31, 48, 92); for reviews see (93, 94).
2) C4d is the cleavage product
of the activated complement component C4, which is activated and degraded as part of the antibody-mediated classic complement pathway.
In order to establish RNAi-mediated effects in vivo, 5'RACE has been established as a robust method for detecting the specific cleavage products
of siRNA pharmacology in pre-clinical animal models (Soutschek et al.
Their test detected only the entire OC molecule and not the more stable cleavage product
are then detected by electrophoretic separation.
DETERMINATION OF THE TEST CONJUGATES AND THEIR CLEAVAGE PRODUCTS IN URINE
Hence, the chemical cleavage yields a fluorescent fragment at the 5' end, whereas the other cleavage product
(3' end) is not detectable by CE-LIF.
Cells within the aggregates also expressed c-peptide, a secretory cleavage product
of insulin, indicative of bonafide production of insulin by the hESC-derived cells.