1B) may correspond to furin/subtilisin-like protease cleavage products
, which would be detected by this antibody (Appendix Fig.
The researchers took a closer look at a key protein in Alzheimer's pathogenisis, APP, and one of its cleavage products
One way of bypassing this lack of information has been introduced as a "processing-independent assay," which simply quantifies 1 in vitro cleavage product
that represents all secreted precursor molecules on a molar basis (31, 48, 92); for reviews see (93, 94).
2) C4d is the cleavage product
of the activated complement component C4, which is activated and degraded as part of the antibody-mediated classic complement pathway.
Their test detected only the entire OC molecule and not the more stable cleavage product
are then detected by electrophoretic separation.
DETERMINATION OF THE TEST CONJUGATES AND THEIR CLEAVAGE PRODUCTS IN URINE
Hence, the chemical cleavage yields a fluorescent fragment at the 5' end, whereas the other cleavage product
(3' end) is not detectable by CE-LIF.