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The iron in the ultrafiltrate was quantified by a chromogenic assay or AAS.
This method uses titration to a given concentration of heparin using either the protamine sulfate neutralization technique or a factor Xa inhibition chromogenic assay.
However, such fragments may still possess significant anti-Xa activity and thus be detected via the chromogenic assay.
28) As an alternative to clotting based tests, the chromogenic assays are specific, but currently lack widespread availability.