(82) Chromogenic assays
need sufficient activation of FVIII and the presence of factor IXa, calcium, and phospholipids.
Calibrated Automated Thrombogram (Hemker method for Thrombin Generation), Staclot[R] Vlla-rTF, Liatest[R] Immunoturbidimetric assays for Fibrin Monomer, C4b-BR Stachrom[R] chromogenic assays
for Heparin Cofactor II and PAI-1, Asserachrom[R] ELISA assays for Protein Z, Soluble GPV, FVIhAg, F X: Ag, PAI-1, APA Ig G, M, (32-GP1 IgG IgM, Total and Free TFPI, sEPCR, Thrombomodulin, FVIILAg, PIVKA-II, and a complete offering of Biocytex products for flow cytometry including VASP/P2Y12, Cellquant PNH, and Redquant PNH
For the anti-Xa inhibitors rivaroxaban and apixaban, chromogenic assays
against human factor Xa are preferred.
also the use of chromogenic assays
to replace clotting assays ...
is intended for coagulation tests based on fibrin endpoint detection by optical measurement of the initial rate of change in absorption when performing chromogenic assays
. It is a fully automated, random-access coagulation analyzer capable of performing plasma-based PT, aPTT, and kinetic fibrinogen assays simultaneously and directly on a single, disposable cuvette without additional setup or reagents.
By avoiding this interference, macrosubstrates may substantially improve the performance of chromogenic assays
for measuring functional activities of coagulation factors, fibrinolytic components, complement factors, inhibitors of physiological proteinases, and cofactors of inhibitors such as heparin.
The Sysmex CA-6000 automated coagulation analyzer (Dade International) is a new random access instrument that is capable of performing 20 clot-based and chromogenic assays
. The purpose of this study is to determine whether the CA-6000 demonstrates performance characteristics for prothrombin time (PT), derived fibrinogen (DF), activated partial thromboplastin times (aPTT), fibrinogen (FBG), thrombin time (TT), factors VII (FVII) and VIII (FVIII) activity, antithrombin (AT), and plasminogen (PLG) similar to an established coagulation analyzer, the MLA 1000C (Medical Laboratory Automation).