inhibitory activity of ethanol and DCM : M bark extracts of Ceylon cinnamon is given in Table 2.
We determined the amount of free and esterified cholesterol (total cholesterol) using a colorimetric method (Roche) in the presence of cholesterol oxidase and cholesterol esterase
and then measured the absorbance at 405 nm.
Plasma was assayed for glucose (glucose oxidase), triglycerides (lipase and glycerol kinase), cholesterol (cholesterol esterase
and cholesterol oxidase), HDL-cholesterol (precipitated with phosphotungestic acid), uric acid (uricase), urea (urease) albumin (bromcrasol green), creatinine (picric acid), and total protein (cupric ion), using enzymatic kits (Human Gesellschaft for Biochemical und Diagnostica GmbH, Germany).
One of those enzymes, cholesterol esterase
, softens the polymers, or resins, used in dental work, Shiu Heman Tsang of the University of Toronto in Ontario reported last week at the American Association for Dental Research in San Antonio.
Fasting plasma glucose, cholesterol, and triglycerides were measured using GOD-PAP, CHOD-PAP, and GPO-PAP method on Selectra-ProM, while (measured LDLc) mLDLc and HDLc were measured by cholesterol esterase
method on ADVIA 1800 Chemistry System, respectively.
hydrolysis esterified cholesterol to free cholesterol.
In first step, when a sample mixed with reagent 1 (MES buffer, ph 6.3, detergent 1, cholesterol esterase
, cholesterol oxidase, peroxidase, 4-Amino-Antipyrine and ascorbic acid), non LDL lipoproteins are solubilized by detergent one and release cholesterol is subject to enzymatic reaction to be eliminated.
For cholesterol, the assay consisted of an enzymatic, calorimetric method whereby cholesterol esters were cleaved by the action of cholesterol esterase
to yield free fatty acids and cholesterol.
(EC188.8.131.52 Pseudomonas sp.) was obtained from Asahi Kasei Pharma, cholesterol oxidase (EC184.108.40.206 microorganism) and peroxidase (EC220.127.116.11 Horseradish) from Toyobo, and catalase (EC18.104.22.168 Corynebacterium glutamicum) from Roche Diagnostics.
Quantitative determination of total cholesterol was done by enzymatic cholesterol esterase
The main objective of this work was to prepare an enzymatic membrane where, cholesterol oxidase and cholesterol esterase
were immobilized for the determination of cholesterol in serum.
We used cholesterol esterase
(CHER) (EC22.214.171.124: 134 kDa, Pseudomonas species) from Toyobo, cholesterol oxidase (CHOD) (EC126.96.36.199: 58 kDa, recombinant Escherichia coh) from Kyowa Hakko, PL-D (EC188.8.131.52: 46 kDa, Streptomyces species) from Asahi Kasei, Good's buffer MOPS from Dojindo Laboratories, 4-aminoantipyrine from Kanto Chemical, and N-ethyl-N-(3-methylphenyl)-N'-succinylethylene-diamine manufactured by Kyowa Medex.