We assessed the accuracy of the albumin cobalt binding test
for detecting ischemia in SSc patients and investigated the roles of myocardial ischemia and peripheral oxidative stress in this condition.
This sentinel publication led to a flurry of proposed binding tests
, most of which were nonspecific competitive-binding assays, until 1974, when Jacobsen and Wennberg presented a method for measuring Bf in infant plasma that was based on the observation that only unbound bilirubin serves as a substrate for enzymatic oxidation by horseradish peroxidase (2).
With this proposal, the Commission has renounced a series of relatively binding tests
set out by Directive 2003/102 - tests which were supported by active associations in road protection - and replaces them with alternative measures, such as breaking assistance systems.