One real benefit of these systems, particularly of the organotypic in vitro preparations, is that the manifestation of toxicity can be extrapolated immediately to the manifestation in vivo; for example, corneal damage in the enucleated eye corresponds directly to potential corneal damage in vivo (although it must be recognized that these assays
do not address the key parameter of recovery), or a neural tube defect in whole-embryo culture is expected to predict the potential for a limb defect in vivo.
For 12 samples positive for both assays
, 90% and 50% inhibitory concentration (I[C.
On these grounds, Ennever says, the Ames test would not surpass the other three assays
in the SCIENCE paper.
Chapter 11 Assays
based on the integrated system properties of cells.
To obtain FMF approval, PerkinElmer was required to provide clinical data to prove the clinical validity of the assays
, to demonstrate a commitment to prenatal screening, and to provide evidence of the successful use of assays
in respected international studies.
His main interests are translational research to develop novel diagnostic assays
based on physiological and immune host responses, tools for clinical monitoring of immune reconstitution, and broad-spectrum immunostimulants.
that have attempted to use direct analysis in matrices have previously failed.
The definitive respiratory virus culture testing can require up to two days and most PCR-based tests are either commercially unavailable for certain viruses or hospital laboratories do not have the technical staff and specialized equipment required to develop home-brew assays
Two days later, the New York City Department of Health reported the following: results of the direct fluorescent-antibody (DFA) assay
, using fluorescein-labeled monoclonal antibodies specific to the B.
Brevetoxin activity determined by receptor assay
increased to 25 [+ or -] 7.
PathHunter[TM] [eth]-Arrestin Assays
are the first chemiluminescent GPCR assays
that measure the activation of Gi, Gs and Gq coupled receptor signaling by direct arrestin recruitment--rather than through a secondary messenger.
Using a reporter gene assay
, we conducted initial characterization of the ATP receptor through analog specificity studies using P2X receptor agonists and antagonists with differing receptor subtype selectivity.