Heparinplasma samples were assayed
according to the manufacturer's instructions.
The cells were incubated in the presence or absence of increasing concentrations of [E.sub.2] or the environmental chemicals for 12 hr; extracts were then assayed
for luciferase activity.
Because of the extremely sensitive assay concept, the EDTA-whole blood samples used in the correlation studies were diluted 400-fold before being assayed
to cover a CRP concentration range of 0.064-1200 mg/L.
In subsequent studies, UC 1 was assayed
at 20, 50, 100, 150, and 200 [micro]L, and UC 2 was assayed
at 20, 40, 50, 60, 80, and 100 [micor]L; protein concentrations were derived by extrapolation of the absorbance values from a calibration curve.
The samples were assayed
along side patient samples and the Amplicor HCV Monitor kit positive control in regular clinical runs for HCV viral load determination.
Serum samples from 15 AMI patients collected at different times after their arrival at the hospital for chest pain were assayed
simultaneously with the three assays calibrated with the TIC complex.
To avoid the questions of sample stability and drug metabolism, FK-506 was assayed
the same day by both methods.