Immunofluorescence assay Tests in which antigen-antibody reactions
are detected by fluorescence because one of the reactants is tagged with a fluorescent dye.
The test kit--developed by Idetek, Inc., of San Bruno, Calif., and based on work done at the USDA's Agricultural Research Service in Beltsville, Md.--uses an antigen-antibody reaction
(2) Anti-human globulin (AHG) is then added to produce an antigen-antibody reaction
. If no agglutination is observed (negative antibody screen), then it can be concluded that no significant antibodies are present.
On getting these encouraging results, a test kit was developed by coating the ~40kDa epitope on a nitrocellulose membrane strip (Millipore HF Plus 135) and detected specific antigen-antibody reaction
by chromogenic assay (colloidal Gold conjugated human immunoglobulin).
When standard adsorption studies are required, numerous technical procedures are employed nationwide, which include untreated RBCs without antigen-antibody reaction
potentiators, untreated RBCs with potentiators such as low ionic strength solutions (LISS) or polyethylene glycol (PEG), enzymatic treatment of RBCs without potentiators, and enzyme treated RBCs in conjunction with various potentiators.