Mid-trimester foetal blood-derived adherent cells
share characteristics similar to mesenchymal stem cells but full-term umbilical cord blood does not.
Sartorius Stedim Biotech launched a new mini bioreactor vessel for its ambr 250 high-throughput (ht) system, designed for optimal growth of adherent cells
on microcarriers to enable rapid, scalable cell culture process development of vaccines.
Suspended and adherent cells
were collected on day 12 from the medium and analyzed by flow cytometry for the further downstream processing.
Both oxidases showed distinct positive reactions in cytospin and adherent cell
preparations (see Table 1), both oxidases labeling similar cell types: spherical cells filled with small, round granules, and amorphous hyaline cells with granules interspersed in the cytoplasm.
Table 3 shows the footprint of the clean rooms and media volumes needed for culturing of different lot sizes of adherent cell
therapy in 3D bioreactors.
More than 92.88% of the adherent cell
population was M1 macrophages as determined by specific surface marker CD11b and CD86 staining after incubation with LPS.
Accutase was gently perfused into the microtube and allowed to incubate for 10 min to detach adherent cells
, then medium was perfused through the microtube to elute cells.
Patrick Mahieux, general manager of ABL Europe, said, "The introduction of non-adherent cell culture capacity complements our existing adherent cell
culture viral vector production technologies.
The CellectCell MicroCassette[TM] is used to rapidly isolate many different adherent cell
types, including mesenchymal stem cells, induced pluripotent stem cells (iPSCs), neural cells, cardiac cells, fibroblasts and cancer cells.
EVANSTON, Ill., January 13, 2015 -- Researchers have developed a device that creates nanopores in adherent cell
membranes, allowing delivery of molecules directly into the cells during differentiation.
This low-binding microplate surface suppresses the adhesion of a variety of adherent cell
lines, can enhance the formation and maintenance of uniform spheroid cultures and can inhibit early differentiation of stern cells or neurospheres.
Integrity[TM] Xpansion[TM] Bioreactor Systems have been specifically developed for fragile adherent cell
culture applications, such as stem cells, on a large scale.