Use of ELISA for Detection of VTG
from Panopea japonica
Although, some subunits of Vtgs
are highly conserved and share a common general structure among fish (Finn, 2007), its complexity due to posttranslational modifications and translocation processes, often results, in poor antigenic cross-reactivity, even between closely related species (Watts et al., 2003; An et al., 2007).
In the liver of males treated with FSH followed by Clomiphene exposure, no positivity to ER[alpha] (Figure 4(d)) neither VTG
antibodies (Figure 4(h)) was detected.
In the present study, the levels of vtg
mRNA altered in juvenile fish exposed to 10, 100 and 1000g/l BPA for 14 days.
Prowse and Byrne (2012) reported that the sea stars Parvulastra exigua and Patiriella regularis contain transcripts for a true Vtg
, the first such report.
The expression of VTG
mRNA and VTG
protein in the liver is shown in Figure 4.
A small section of each liver was placed in RNAlater (Qiagen, Valencia, CA, USA) and stored at-80[degrees]C for analysis of vtg
expression as described below.
It is recommended that the term panting itself should not be used and, most often, the best instructions are to ask the patient to breath normally during the both the Vtg
and Raw measurements.
Nevertheless, the 140 unit boosted by the VTG
turbocharger, ensures a spirited enough drive, with a o-62mph in just 9.4 seconds and a top speed of I29mph.
To diagnose endocrine disruption, we measured sex steroid hormone [17[beta]-estradiol ([E.sub.2]), testosterone (T), and 11-ketotestosterone (11KT)] and vitellogenin (VTG
) concentrations in male and female longear sunfish captured at two sites upstream and two sites downstream of the effluent outfall.