lines were propagated in Dulbecco's minimal essential medium (DMEM; Gibco-BRL, Grand Island, NY) enriched with 10% calf serum (CS; Gibco-BRL) and antibiotics.
At the end of 48 hours, Al values were found as 4% for the control group and 29% for the experiment group of Vero cell
; 4% for the control group and 77% for the experimental group of C-4 I cell (Figure 12).
Cytotoxicity tests of whole cultures and cell-free supernatants of Alexandrium catenella and Alexandrium affine at different growth phases [exponential growth phase (EG), SG, and DP] were conducted on Vero cells
(African green monkey kidney cells), after dilution of the culture and supernatant (1:100) based on previously described methods (Oda et al.
SNO Concentration No: of Cell ([micro]g/ml) cells counted Viability (%) 1 1000 41 19.8 2 500 63 30.43 3 250 75 36.23 4 125 90 43.47 5 Control 207 100 Table 3: Cytotoxicity effect of Graphene Oxide on VERO Cell
A: There was a significant decrease in Vero cell
The present study scored the viability of VERO cell
line were 58.3% at concentration of CSNPs (50^g/ml).
The remainder was held on ice for later use as a control in the Vero cell
The Vero cell
culture obtained from the cell bank of Pasteur Institute (Tehran, Iran) was grown in Dulbecco minimal essential medium in 25 [cm.sup.2] flasks (Nunc, Denmark) under 5% C[O.sub.2] at 36[degrees]C for 48 hours.
To meet this challenge, our research was under taken to evaluate the antibody level against Newcastle disease by using Vero cell
adapted live attenuated thermostable Newcastle disease viral strain I-2 in the local environment of selected region of Pakistan.
The IBH-HPS virus was successfully adapted after three blind passages (Vero cell
line) and the 4th passage was fully adapted as it gave cytopathogenic effect (CPE) in 48 h post infection in Vero cells
The CVA16 strains CC024, CC045, CC090 and CC163 that were isolated in 2010 from throat swabs of hospitalized HFMD patients in Changchun were propagated in a Vero cell