These structures are difficult to identify using a hematoxylin-eosin stain and are best visualized with silver stains
or immunohistochemistry for tau protein.
Although we did not confirm CSF leakage with invasive procedures, we interpreted the two discordant samples as illustrating the lack of sensitivity of the immunofixation/ silver stain
method (method 1) compared with the other methods as well as the difficulty of the nephelometric procedure for classifying borderline abnormal results.
shows double contours, reflecting mesangial cell interposition with the production of extraneous GBM-like material, referred to as tram-tracking, splitting, or duplication of the GBM in both types-I and -II MPGN.
The confirmation of the absence of any contamination of membrane proteins in the purified SGPs (Figure 3A and C, lane 2) was done by silver stain
(Figure 3C, lane 2) which is visualizing only the purified SGPs in the gel.
Silver staining was done with the Bio-Rad Silver Stain
Gommori's methenamine silver stain
was positive (Fig: 6).
Although the patient in our case was not known to have AIDS at the time of diagnosis, the microscopic findings of the granular, frothy, pink material and associated granulomatous feature prompted a test with Grocott methenamine silver stain
Silver staining of the polyacrylamide gel was performed using Bio-Rad silver staining kit following the instructions as described by the Bio-Rad Silver Stain
Handbook with slight modification as fixing the gel in 7.
The Bodian silver stain
method allows visualization of neural tissue in many species (Bodian, 1936; Gambetti et al.
Limited Tenders are invited for Isopropyl Alcohol For Hplc Grade Methanol Hplc Grade Proteosilver Silver Stain
Kit Protsil1-1Kt Mes Monohydrate Ph Range 5.
4) Therefore, in the algorithmic approach for the evaluation of these diseases, the silver stain
plays an important role in determining the diagnostic pathway to follow to fully characterize the disorder in question.
protein, Hoechst, Krypton, silver stain
, copper stain, zinc stain, Coomassie