Splenic sinuses are open vascular spaces lined by a discontinuous layer of endothelial cells and supported by a fenestrated basal lamina and reticular fibers. The surrounding cellular splenic cords provide a tissue frame work maintaining the network of sinuses (Fig.
The paraffin sections were stained with haematoxylin and eosin for routine morphology, Masson's Trichrome for connective tissue, Verhoeff's stain for elastic fibers, and Gridley's stain for reticular fibers.
In control group, normal hepatic cells are characterized by well defined cell linings, prominent nucleus, and prominient central vein surrounded by reticular fibers (Figure 3(a)).