We further investigated the reproducibility of this finding in 16 MRSA strains isolated four years later in 2013, and found that the 3-, 4- and 5-primer MLVA
amplification clustered the tested strains in a similar manner (Figure 2).
Additionally, because of the low cost and powerful discriminatory power of the MLVA
14Orsay genotyping approach, we were able to successfully apply this tool to identify the circulating clones of S.
In the MLVA
results, we found that patients infected with a Beijing family strain were more likely to be clustered than patients who were infected with a non-Beijing family strain.
A confirmed case was defined as illness in a person with an STEC O157 isolate indistinguishable from both PFGE pattern 2 and MLVA
pattern 2 with an isolation date on or after July 1.
The results of our study can be compared with previous studies using MLST and MLVA
. Brown et al.
We extracted MLVA
profiles from whole-genome sequence assemblies.
The relative contributions of common definitive phage types (DT) and MLVA
types to local Salmonella Typhimurium activity varied over 8 seasonal peaks.
anthracis of MLVA
genotype 1, a different genotype than the Ames strain used in the 2001 mail-related anthrax attacks (1).
Evaluation and selection of tandem repeat loci for a Brucella MLVA
A list of loci were targeted for strain typing (3,4) and multi locus variable number tandem repeat analysis (MLVA
) as means for molecular differentiation of M.
pestis isolates from patient 1 and flea samples were compared by using pulsed-field gel electrophoresis (PFGE) and multiple locus variable number tandem repeat assay (MLVA
) sequences (1).
We characterized 56 strains at Institut Pasteur (Paris, France) by determining the antimicrobial drug resistance patterns using the disk diffusion method following CA-SFM (Comite de l'Antibiogramme de la Societe Francaise de Microbiologie) 2013 standards for Enterobacteriaceae (http://www.sfm-microbiologie.org/); subtyping with pulse-field gel electrophoresis (8) with SfiI and NotI restriction enzymes (Roche Molecular Biochemicals, Indianapolis, IN, USA); and multilocus variable number tandem repeat (MLVA
) analysis targeting 6 loci in the V.