Practical considerations in refolding proteins from inclusion bodies
. Protein Expr Purif.
coli showed that the recombinant BMP-2 was exclusively expressed as an insoluble fraction and accumulated in cytoplasm as inclusion bodies
. Inclusion body extracted proteins exhibited a sharp band between 17 to 20 kDa, corresponding to monomeric chain of BMP-2 (Figure 3).
Cells stained with hematoxylin and eosin contained large eosinophilic cytoplasmic inclusion bodies
that were compatible with infection by poxviruses, including CPXV (36) and ECTV (37) (Figure 2, panel B).
SDS-PAGE analysis of expressed protein indicated that almost 95% of the AMYSBS was produced in the form of inclusion bodies
(lane 3, Fig.
Prominent microscopic lesions in lymphoid organs included intra cytoplasmic inclusion bodies
, syncytial cell formation, depletion and necrosis of lymphoid tissues with numerous lymphocyte infiltration with pyknotic nuclei, seen from day 14 to 21 of infection in untreated group B (Plate 3).
The patient remained asymptomatic on follow-up, with improved appetite; repeat ampullary biopsy showed normal architecture with no evidence of CMV inclusion bodies
. In all the previous cases of IRIS secondary to CMV, the patients were treated with either ganciclovir or valganciclovir.
Despite the optimization of the expression conditions for pearlin, it was mainly expressed in the form of inclusion bodies
that needed to be solubilized in harsh conditions; renaturation and affinity purification were then used to obtain the physiologically active protein.
When HBH preparation was done for such patients, numerous inclusion bodies
were observed on slide (Fig.3).
Caption: Figure 4: Liver biopsy showing the periportal localization of periodic acid-Schiff positive inclusion bodies
, confirming the diagnosis of [alpha.sub.1] antitrypsin deficiency.
Though these skin manifestations are heterogeneous, they all exhibit a typical histological appearance that includes vascular endothelial cytopathic changes and intranuclear inclusion bodies
There were multiple vacuoles containing chlamydial inclusion bodies
at different stages of life cycle (Figures 5(a) and 5(b)) with typical elementary and reticulate bodies.
(6) Originally, it was unknown what these inclusion bodies