Relative sensitivity of direct antiglobulin test
, antibody's elution and flow cytometry in the serologic diagnosis of immune hemolytic transfusion reactions.
Else et al., "The prognostic significance of a positive direct antiglobulin test
in chronic lymphocytic leukemia: a beneficial effect of the combination of fludarabine and cyclophosphamide on the incidence of hemolytic anemia," Blood, vol.
Likewise, Umit et al had opined that a positive direct antiglobulin test
was a good predictor for severe ABO haemolytic disease.
Direct Antiglobulin Test
Antiglobulin Test 1 Y K1 2 Y Positive for autoantibody 3 N Lea 4 Y History of DAT positivity Fya and autoantibody (autoantibody) 5 Y Positive for passive Negative anti-D 6 Y History of DAT positivity Negative (autoantibody) 7 N Positive for passive Passive anti-D and anti-D cold antibody 8 N Lea 9 Y Lea 10 N C 11 N D, E 12 N Positive for autoantibody Anti-E, DHTR vs DSTR discovered 13 N D 14 N K1 15 N Cold antibody, roleaux 16 Y History of DAT positivity Negative (autoantibody) 17 Y History of DAT positivity Negative (passive anti-D and autoantibody) 18 Y Positive for autoantibody E, C, Fyb, and autoantibody 19 N K1, S 20 Y Leb 21 Y Positive for passive D anti-D 22 Y D 23 N D due to passive anti-D Hemolytic Blood Case Potential?
A detailed clinical and transfusion history was taken using a set performa which included the name, identification number, age, sex, diagnosis, blood group, transfusions done till date of request, transfusions during the present study period, result of serological testing like direct antiglobulin test
and auto control, antibody screen tests in the study period with results and antibody identification results.
The initial symptoms and laboratory findings of hemolytic anemia, depressed hemoglobin and haptoglobin concentrations, increased lactate dehydrogenase, increased indirect bilirubin, and an IgG-positive result in a direct antiglobulin test
were all consistent with those typically indicative of warm antibody AIHA, although the reticulocytosis often also associated with AIHA was not noted (1).
Serum antibody screens and the direct antiglobulin test
Results of the patient's direct antiglobulin test
(DAT) were strongly positive using broad-spectrum reagents and anti-immunoglobulin G (IgG) and C3 antibodies.