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The manual specimen preparation method isolates HCV RNA from 200 [micro]L of plasma by lysis of the virus with a chaotropic agent, guanidine thiocyanate, followed by precipitation of the RNA with isopropyl alcohol.
Given our observed inability to greatly alter soy flour adhesion using chaotropic agents, salts, surfactants, or co-solvents (Frihart and Lorenz 2013), which is in contrast to the literature observations with soy protein isolate (Sun 2005b), we concluded that the carbohydrates were suppressing protein alteration for improved adhesion.
The list of substances which have been shown to inhibit DNA polymerases is long, but some of the most commonly encountered problem substances in clinical specimens include heme (and thus whole blood); chelating agents (including many anticoagulants, whose mode of action is by Ca++/Mg++ chelation; removal of these from the polymerase buffer makes the dNTPs less reactive); ethanol or isopropanol; and protein denaturing or chaotropic agents such as guanidine or urea.