19) However, our review of the literature has failed to reveal any study in which the use of CZE
has been studied for birds.
In conclusion, the administration of large amounts of Geloplasma can cause important CZE
interference in the [beta]/[gamma]-region, which can be confused with an M-protein in patient samples.
For samples from both patients, CZE
revealed suspect peaks in the (3 fraction after angiocardiography that were clearly discernable up to 8 h after angiocardiography in the first patient and up to 4 h in the second patient.
No PA-induced modification of the CZE
pattern was apparent visually in samples not containing IgM MCs.
For both CZE
and HPLC, CDT isoform quantification (%CDT) was based on calculation of the percentage ratio of the (asialo-Tf + disialo-Tf) peak area to the sum of the peak areas for all isotransferrins from asialo-Tf to pentasialo-Tf.
Although the detection of low-concentration monoclonal heavy chains by CZE
has been reported (1), the present report demonstrates that some heavy chain diseases are missed by CZE
This may lead to diagnostic confusion in instances where blood for SPE by CZE
is taken soon after an imaging procedure or in patients in whom renal clearance of contrast agent is defective.
Problems with the detection of monoclonal components by CZE
have been described previously (3-5), and a report similar to this one has appeared (6).
When we used ethanol precipitation to treat seven serum samples with suspected fibrinogen peaks, we found that these peaks disappeared on CZE
in only three of the samples.
After receiving approval by our Institutional Review Board and the patients' informed consent, we evaluated the in vivo effect of Gelofusine on CZE
analysis of samples from patients (n = 10) who had received 500-mL infusions of Gelofusine over 1 h for hemodilution during orthopedic surgery by comparing the electropherograms for samples obtained before and immediately after the infusion.
Collectively, the recently introduced new buffer system for CZE
with the Paragon CZE
system has a slightly increased sensitivity for detecting M-proteins that were previously missed.
1 are CZE
electropherograms (Beckman Coulter Paragon CZE
2000, software Ver.