For immunoprecipitation studies, mouse monoclonal antibody against human CREBBP (C-1, 20 [micro]g; Santa Cruz Biotechnology) was incubated with 10 [micro]l of protein A Sepharose beads (Amersham Pharmacia) at 4[degrees]C for 4 h (Imakawa et al.
Similar levels of transcription factor CREBBP, JUN, ETS2 mRNAs were found this time period whereas IFNT mRNA declined (Figure 1A).
, a gene involved in the Sri-lad pathway, was significantly up-regulated by NF3, which has been demonstrated to be a necessary co-activator for the Smad-dependent TGF-[beta] transcriptional response (Topper et al.
elegans) (EGLN3); E1A binding protein p300 (EP300); and CREB binding protein (CREBBP
), whereas miR-200b, miR-200c, and miR-429 target VHL; transcription elongation factor B (SIII), polypeptide 1 (15 kDa, elongin C) (TCEB1); EGLN1/3; EP300; and CREBBP