For phenotypic plot, subpopulations of [CD4.sup.+] T cells (Figures 1(a) and 1(b)) and [CD8.sup.+] T cells (Figures 1(c) and 1(d)) are characterized using the mAb combination of CD45RA with CD62L
compared to the one of CD45RA with CCR7.
5 depicts a flow cytometer dot plot, showing uniform expression of CD16 and CD62L
by normal granulocytes before and after LPS administration.
Both lung- and bone marrow-purified [CD11b.sup.+][Gr-1.sup.+] and [CD11b.sup.+] [Gr-1.sup.-] myeloid cells were stained for CD80, CD86, MHCII, CD62L
, and F4/80 for flow cytometry analysis.
Flow cytometry was used to determine the proportions of [CD4.sup.+], [CD5.sup.+], [CD8.sup.+], [CD21.sup.+], [CD25.sup.+] lymphocytes and co-expressing CD25 and CD62L
in [CD4.sup.+] and [CD8.sup.+] T cells in PBMC.
The leukocyte isoform L-selectin (CD62L
) is an 80-kDa molecule produced on the surfaces of resting human neutrophils.
Biomarkers of neutrophil activation included L-selectin (CD62L
), intercellular adhesion molecule-1 (ICAM-1, CD54), and matrix metalloproteinase-9 (MMP-9, gelatinase B) (R&D Systems, Minneapolis, MN, USA), as well as IL-8 (as mentioned above), the levels of which were determined by conventional ELISA kits.
Salah, "Regulatory T cells with CD62L
or TNFR2 expression in young type 1 diabetic patients: relation to inflammation, glycemic control and micro-vascular complications," Journal of Diabetes and Its Complications, vol.
They found that insulin increased the total number of neutrophils and the number of these expressing CD11b, CD15, CD62L
, and CD89, whereas the density of these molecules was downregulated.
Expression of CD62L
showed a significant exposure-sex interaction (p = 0.0006; data not shown), with expression increasing in females but decreasing in males relative to air exposure.
E-cadherin, an adhesion molecule mainly found on the surface of epithelial cells,[11,14] has been demonstrated to play major roles during morphogenesis, in the maintenance of epithelial tissues, and in malignant tumors. It was recently demonstrated by flow cytometric analysis that E-cadherin is transiently expressed on bone marrow erythropoietic cells.[6,17] Double-labeling experiments with anti-E-cadherin antibody revealed coexpression with the molecules glycophorin A and the transferrin receptor, while no coexpression was found with lymphoid (CD2, CD3, CD10, CD19, CD56, and CD62L
) and myeloid (CD13, CD33, and CD41) markers.[6,17] E-cadherin appeared to be strongly expressed on erythroblasts and to decrease during maturation of erythroid precursors.
Monoclonal antibodies (mAbs) with specificity against CD4 (RM4-4), CD25 (7D4), CD62L
(Mel-14), and CTLA4 (UC10-4F10-11) were used.