2.5 In vitro bacterial reverse gene mutation test (Ames test
Endpoint specific Structural alerts profilers Bisoprolol Impurity API RRT 0.95 Carcinogenicity No alert No alert (genotoxic and found found nongenotoxic) by ISS DNA alerts for No alert No alert Ames by found found OASIS DNA alerts for No alert No alert CA and MNT found found by OASIS In vitro No alert No alert mutagenicity found found (Ames test
) by ISS In vivo H-acceptor- H-acceptor- chromosomal path3-H- path3-H- mutation acceptor acceptor OncoLogic No alert No alert primary found found classification
More than one assay should be conducted and due to Ames test
limitations it should not be a standalone study in the tier.
According to the risk analysis principle, it is the task of the scientist to perform the risk assessment as a basis for differentiating between mushrooms suitable to be marketed as food mushrooms and those are not by using Ames test
to detect mutagenic compound in mushrooms as described by Jorn and Anderson .
Castela texana, an antiamebic plant, was shown not to be mutagenic in the Ames test
; in contrast, it is an antioxidant and reduces mutations induced by fluoroquinolones .
Biomonitoring of the genotoxic potential of draining water from dredged sediments, Using the comet and micronucleus tests On amphibian (Xenopuslcevis) larvae and Bacterial assays (mutatox[R] and ames tests
Thus, the SOS-Inductest detects agents that inhibit DNA replication (genotoxic agents), while the Ames test
is effective to identify genetic damage that leads to gene mutations (mutagenic compounds).
Genotoxicity of hazardous leachates from solid wastes evaluated for environmental impact with the Ames test
. Environ Toxicol Water Qual 7:71-86.
"Instead the FDA wants the Ames test
plus either mouse lymphoma or chromosome aberration plus a further test, mouse micronucleus.
Assay for in vitro-in vivo mutagenicity of fecal and urinary samples by Ames test
Other presentations in the DDP conference included: "Preventing (or Accelerating) Aging and Degenerative Disease," by Bruce Ames, Ph.D., inventor of the Ames test
for mutagenicity and professor of biochemistry and molecular biology at U.C.
In the Ames test
, not all substances that cause mutations in bacteria are found to cause cancer in animals or man.