Considering the high homology between P-type ATPases
, it is possible that PPIs can inhibit other ionic pumps in different organs or even induce systemic physiological changes.
It was reported that although a chemical modification of Tyr307 of single [beta] in [F.sub.1] suppressed ATPase
activity for T[F.sub.1] and E[F.sub.1], (48,49) a simple mutation such as Y307C did not impair the ATPase
activity, (50) suggesting that the ring itself was not an essential element for the activity.
The decreased activity of ATPase
may reduce the transport capacity of [Ca.sup.2+], and then result in the formation of intracellular and extracellular [Ca.sup.2+] concentration gradient as well as the balance of [Ca.sup.2+] pump in the cytosol.
The vast majority of mutations causing NCBRS are located in the ATPase
domains of exons 15-25 of this gene [9,16,17]; however, atypical variants outside the ATP domain have also been described in individuals with typical NCBRS, highlighting the broad spectrum of the syndrome and helping to elucidate more genotypes and phenotypes .
Here, we demonstrated that endurance exercise training promoted important adaptations in skeletal muscle by increasing protein content of PGC-1[alpha] and PDK-4, and enzyme activity of citrate synthase and myosin ATPase
. Also, in the liver, elevated citrate synthase activity and cytochrome C content, proteins related to mitochondrial oxidative capacity and phosphorylation processes, were observed, and elevated acetyl-CoA carboxylase phosphorylation, suggesting a reduced de novo lipogenesis.
In vivo insulin effect on ATPase
activities in erythrocyte membrane from insulin-dependent diabetics.
activity was determined using the SB-MDR1 PREADEASY ATPase
Kit (SOLVO Biotechnology, Szeged, Hungary) according to the manufacturer's instructions.
To evaluate the effects of Ang II on [Na.sup.+]/[K.sup.+]/ ATPase
subunits expression, the morula and blastocyst embryos on day 8 were immunostained with different specific primary and a common Fluorescein Isothiocyanate (FITC)-conjugated secondary antibodies.
Lipids modulation of plasma membrane-bound ATPase
. Physiologia Plantarum 78: 153-159.
activity was measures in a final volume of 1 ml as follows: Membranes (400ug) were preincubated for 10 min.
The [Na.sup.+]/[K.sup.+] ATPase
pump (Na/K pump) in neurons plays a key role in maintaining the transmembrane electrical gradient that is critical for normal function.
The F1 domain of ATPase
contains the catalytic site for ATP synthesis and hydrolysis and the binding sites for ATP and ADP .