Effect of rare variants in ADRB2
on risk of severe exacerbations and symptom control during longacting b agonist treatment in a multiethnic asthma population: a genetic study.
The horse ADRB2
transcript was analyzed by real-time polymerase chain reaction (RT-PCR) amplification.
We have previously described a noncontinuous probe that spans 3 loci of the ADRB2
gene and determines the 3 main haplotypes of the gene (22).
Twelve genes were selected from among genotyped MESA candidate genes: ACE, ADRB2
, AGT, AGTR1, ALOX15, EDN1, GRK4, PTGS1, PTGS2, TLR4, VEGFA, and VEGFB.
The SNP genotypes were determined by probe-extension reactions using two probes with different base sequences at their 3' termini: rs1042718 on the ADRB2
gene (5'-CCC ATT CAG ATG CAC TGG TTC N-3', where N = A or C); rs3918242 on the gelatinase B gene (5'-CTC CCG AGT AGC TGG TAT TAT AGA CN-3', where N = G or A); rs1346044 on the WRN (5'-CTC CTT TTG TTG ACA TCT CN-3', where N = A or G); and rs1801133 on the MTHFR gene (5'-CTT GAA GGA GAA GGT GTC TGC GGG TGN-3', where N = C or T).
However, a previous study evaluating the combined effects of air pollutants and ADRB2
in children did not report significant findings (Melen et al.