A complete ocular examination including modified Schirmer tear test I (mSTTI) (Schirmer tear test, Intervet, Summit, NJ, USA) (n = 9 birds; 18 eyes) or phenol red
thread test (PRTT) (Zone-Quick, Showa Yakuhin Kako Co, Tokyo, Japan) (n = 8 birds; 16 eyes), fluorescein staining, and intraocular pressure (IOP) evaluation by applanation (Tonopen XL [TP] Mentor Corp, Santa Barbara, CA, USA) (n = 9 birds; 18 eyes) or rebound (TonoVet [TV] Tiolat Oy Lumic International, Baltimore, MD, USA) (n = 8 birds; 16 eyes) tonometry followed by slit-lamp biomicroscopy, and direct ophthalmoscopy was performed.
Measurement of secretolytic activity was evaluated by measuring the excretion of phenol red
into respiratory tract (Coppi and Gatti 1989).
dextrose broth (PRDB) was tested as a potential metabolic inhibition test (MIT) and selective medium, when supplemented with antibiotics to differentiate bacterial phenotypes.
Key words: eye, tear production, PRTT, phenol red
thread test, avian, common mynah, A cridotheres tristis
GE was determined after a modification of the phenol red
method (Tache et al.
Key words: phenol red
thread test, tear, ophthalmology, Falconiformes, avian, Falco
Each well of a sterile 96-well flat-bottomed, microtitre plate was inoculated with 100 [micro]l of media, one plate with enriched PPLO medium with glucose and phenol red
and the other without.
For strain cultures, cells were grown in phenol red
containing Dulbecco's modified Eagle medium, 1 g/L glucose, supplemented with 5% fetal calf serum (FCS), and 1% antibiotic (penicillin/streptomycin) in a 5% C[O.
thread tear test (PRT) was performed to evaluate tear production as described in a previous study.
mediu sperm washing with phenol red
and gentamicin 50ml = 168-336
Cells for transient transfection assays were seeded in DME-F12 medium without phenol red
and supplemented with 5% dextran-charcoal-stripped FBS, 2.