Assessment of problems in diagnostic and research immunohistochemistry associated with epitope instability in stored paraffin sections
Nevertheless, paraffin section
IHC stains for [kappa], [lambda],and sometimes the immunoglobulin heavy chains are useful in evaluating potential lymphoid or plasmacytic neoplasms, especially if there is plasmacytoid differentiation or overt plasma cells.
The interpretation of staining or its absence was confirmed by subsequent IHC on paraffin sections
together with positive controls.
In paraffin sections
, myeloperoxidase and lysozyme antibodies have been used as sensitive and specific markers for cells of myeloid lineage?
FISH analysis on paraffin sections
shows that heterogeneity of monosomy of chromosome 3 is a frequent phenomenon in uveal melanoma.
Template DNA was extracted from the paraffin sections
of the cecum that contained the botryomycotic granules of the current case, followed by the method described by Hykin et al.
Additional paraffin sections
of selected blocks were obtained for immunohistochemical studies, which were performed on an automated immunostainer (Ventana, Biotek System, Tucson, Ariz) with appropriate positive and negative controls run concurrently.
Four-micrometer-thick paraffin sections
were dewaxed in toluene and hydrated through graded alcohols to distilled water.
The new method is easy and applicable to routine paraffin sections
Twenty-two cases and the following subtypes were retrieved for immunoperoxidase staining of paraffin sections
with CD30: follicular, predominantly small cleaved cells (3 cases); follicular, [+ or -] diffuse, mixed, small cleaved, and large cells (10 cases); follicular, [+ or -] diffuse, large cell (8 cases); and diffuse, large cell (1 case).
Contract notice: Supply, installation and maintenance of a full automatic system for realization of molecular markers by antibodies or other probes on paraffin sections
Currently, Paradigm is the only organization with the ability to interrogate mRNA targets by NGS in formalin fixed paraffin sections
along with the other genomic markers.