Moreover, the DM96 was found to result in similar differential counts
as manual methods.
Our optimized criteria were the same as those of the consensus group criteria for low hemoglobin values, RDW, high WBC counts, low PLT, eosinophil counts, basophil counts, no or incomplete differential counts
, and the presence of suspect flags.
As shown in Table 3, the biological coefficients of variation of total leukocytes and their differential counts
are constant except for the 4 PM time point on day 3.
The results of each 400-cell differential count
were analyzed, and any samples that were not within the 95% confidence interval were arbitrated by a senior member of the laboratory staff.
With high-speed computing technology it is possible to derive a microscopic differential count
on many more cells than the traditional 100 cell count performed by manual microscopy.
Specimens concentrated by centrifugation or cytocentrifugation are used to perform differential counts
and assess cell morphology on body fluids.
5 g/L, differential count
not done if too few leukocytes, Urine M-component not analyzed if U-protein <0.
Kasdan expressed his belief that The White IRIS(TM) heralds the advent of a new era in hematology and anticipates a 500-cell differential count
can be obtained in approximately one minute, as compared to current manual procedures which require six minutes or longer to differentiate and count 100 white cells.
On day 8, the numbers of atypical cells decreased considerably and continued to fall until day 11 when the differential count
registered 2926/[micro]L atypical cells.
The advantages of automated differential count
over manual differentiation using 100 or 200 cells are greater analytical precision, because the differential leukocyte count obtained by the analyzer is performed by counting thousands of cells instead of hundreds and is accompanied by excellent accuracy, at least for neutrophils and lymphocytes, and reduction in costs and turnaround time due to high throughput .
For a differential count
, air-dried cytocentrifuge preparations with Wright's staining are preferred, (1), (2) as the most number of cells can be concentrated from the CSF specimen, and the Wright's stain gives the best morphology.
The DM1200 automatically performs a preliminary differential count
on peripheral blood.