One report (30), in which whole blood was flushed with nitrogen, indicated that assayable RCF may only be increased up to twofold.
One report in the literature (30) (the implications of which appear to have been overlooked) reported that the degree of Hb oxygenation altered RCF amounts such that deoxygenation increased, and oxygenation decreased, assayable folate concentration in both MA and RFBP assays.
On the basis of results from study l(a), it would be expected that assayable RCF for a diluent of 10 g/L AA, pH 6.
4), the assayable RCF concentration when saponin was not added was 81.
0 produced very poor hemolysis and, hence, low assayable RCF concentrations.
3), demonstrated that although sonication for 30 s in an ultrasonic water bath had no significant impact on RCF concentration and, by implication, the degree of hemolysis, subjecting samples to one freeze-thaw cycle before RCF deconjugation increased the assayable folate concentration from 15% to ~100% of the comparative values seen in lysates with added saponin.