ABCG1 is deficient in alveolar macrophages of
GM-CSF knock-out mice and patients with pulmonary alveolar proteinosis.
NO,
GM-CSF, and VEGF play crucial roles in regulating the function of circulating EPCs [23, 24].
Since caloric restriction prevents chronic inflammation, the increased (P<0.05) serum levels of
GM-CSF, IFN-gamma, and KC (HFD vs HFD-FR), represent an unexpected result.
In line with our findings a study by Hill et al26 on patients with CAD indicated that, although
GM-CSF mobilizes endothelial progenitor cells from bone marrow, however, it does not improve cardiac function significantly and conversely, it contributes to some serious adverse events in these patients.
Scott Halstead said that the results suggested that the
GM-CSF could be a potential therapeutic strategy for treating the flu.
Several methods such as bone marrow culturing with either
GM-CSF alone [11, 12] or in combination with IL-4 [6,18], by Flt3L [20, 21] or IL-3 [22], are used for generation of murine BMDCs.
We applied a flow cytometry-based method utilizing magnetic microspheres conjugated with monoclonal antibodies (Luminex xMAP[R] Technology) to measure the concentrations of eotaxin,
GM-CSF, IFNy, IL4, IL5, IL8, IL12(p70), IL13, RANTES, and TNF[alpha].
Due to
GM-CSF and M-CSF being well-known colony-stimulating factors involved in monocyte proliferation and differentiation, levels of mRNA and proteins of
GM-CSF as well as of M-CSF were assessed by real-time PCR and ELISA tests.
Autoimmune PAP, which makes up 90% of reported PAP cases [3], is caused by the production of autoantibodies against
GM-CSF, a crucial cytokine in the maturation of monocytes into macrophages [7].
Concentrations of eight proinflammatory cytokines, IL-2, 4, 6, 8,10,
GM-CSF, IFN-[gamma], and TNF-[alpha] were measured using the Bio-Plex Pro Assays 8-Plex assay kit (Bio-Rad, Cat#M50-0007A) and Bio-Plex Multiplex immunoassay system (Biorad Bio-Plex 200) according to the manufacturer's instruction.
Measurement of Levels of NO, VEGF,
GM-CSF, TNF-[alpha], and IL-6 in Plasma.